ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 2-reagent selection and assay optimization for high-sensitivity testing

被引：49
|
作者：
Sutherland, D. Robert [1 ]
Illingworth, Andrea [2 ]
Marinov, Iuri [3 ]
Ortiz, Fernando [1 ]
Andreasen, John [4 ]
Payne, Dan [5 ]
Wallace, Paul K. [6 ]
Keeney, Michael [7 ]
机构：
[1] Toronto Gen Hosp, Univ Hlth Network, Toronto, ON M5G 2C4, Canada
[2] Dahl Chase Diagnost Serv Bangor, Bangor, ME USA
[3] Inst Hematol & Blood Transfus, Prague, Czech Republic
[4] ARUP Labs Inc, Immunol Flow Cytometry, Salt Lake City, UT USA
[5] Leicester Royal Infirm UHL NHS Trust, HMDL & Immunol Flow Cytometry Serv, Leicester, Leics, England
[6] Roswell Pk Canc Inst, Dept Flow & Image Cytometry, Buffalo, NY 14263 USA
[7] London Hlth Sci Ctr, Dept Hematol Flow Cytometry, London, ON, Canada
来源：
CYTOMETRY PART B-CLINICAL CYTOMETRY | 2018年 / 94卷 / 01期
关键词：
high sensitivity flow cytometry; paroxysmal nocturnal hemoglobinuria; PNH; FLOW-CYTOMETRY; PRACTICAL GUIDELINES; DIAGNOSIS; MONOCYTES; FLAER; CD157;
D O I：
10.1002/cyto.b.21610
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Since publication in 2010 of the International Clinical Cytometry Society (ICCS) Consensus Guidelines for detection of Paroxysmal nocturnal hemoglobinuria (PNH) by flow cytometery, a great deal of work has been performed to develop, optimize, and validate a number of high-sensitivity assays to detect PNH phenotypes in both red blood cells (RBCs) and white blood cells (WBCs, neutrophils, and monocytes). This section (Part 2) of the updated ICCS PNH Consensus Guidelines will focus on specific instrument setup for these PNH assays, the identification and proper testing of appropriate antibody conjugates and combinations therof, and basic assay design. (c) 2017 International Clinical Cytometry Society
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页码：23 / 48
页数：26
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