Field evaluation of a Pan-Lassa rapid diagnostic test during the 2018 Nigerian Lassa fever outbreak

被引：12

作者：

Boisen, Matthew L. ^{[1
]}

Uyigue, Eghosa ^{[2
,3
,4
]}

Aiyepada, John ^{[4
]}

Siddle, Katherine J. ^{[5
,6
,7
]}

Oestereich, Lisa ^{[8
,9
]}

Nelson, Diana K. S. ^{[1
]}

Bush, Duane J. ^{[1
]}

Rowland, Megan M. ^{[1
]}

Heinrich, Megan L. ^{[1
]}

Eromon, Philomena ^{[2
]}

Kayode, Adeyemi T. ^{[2
,3
]}

Odia, Ikponmwosa ^{[4
]}

Adomeh, Donatus, I ^{[4
]}

Muoebonam, Ekene B. ^{[4
]}

Akhilomen, Patience ^{[4
]}

Okonofua, Grace ^{[4
]}

Osiemi, Blessing ^{[4
]}

Omoregie, Omigie ^{[4
]}

Airende, Michael ^{[4
]}

Agbukor, Jacqueline ^{[4
]}

Ehikhametalor, Solomon ^{[4
]}

Aire, Chris Okafi ^{[4
]}

Duraffour, Sophie ^{[8
,9
]}

Pahlmann, Meike ^{[8
,9
]}

Bohm, Wiebke ^{[8
,9
]}

Barnes, Kayla G. ^{[5
,6
,10
]}

Mehta, Samar ^{[5
,6
,11
]}

Momoh, Mambu ^{[12
,13
,14
]}

Sandi, John Demby ^{[13
,14
]}

Goba, Augustine ^{[13
,14
]}

Folarin, Onikepe A. ^{[2
,3
]}

Ogbaini-Emovan, Ephraim ^{[4
]}

Asogun, Danny A. ^{[4
]}

Tobin, Ekaete A. ^{[4
]}

Akpede, George O. ^{[4
]}

Okogbenin, Sylvanus A. ^{[4
]}

Okokhere, Peter O. ^{[4
,15
,16
]}

Grant, Donald S. ^{[13
,14
,17
]}

Schieffelin, John S. ^{[18
,19
]}

Sabeti, Pardis C. ^{[5
,6
,7
,10
,20
,21
]}

Gunther, Stephan ^{[8
,9
]}

Happi, Christian T. ^{[2
,3
,4
,10
]}

Branco, Luis M. ^{[1
]}

Garry, Robert F. ^{[1
,22
,23
]}

机构：

[1] Zalgen Labs LLC, Germantown, MD 20876 USA

[2] Redeemers Univ, African Ctr Excellence Genom Infect Dis, Ede, Osun State, Nigeria

[3] Redeemers Univ, Coll Nat Sci, Dept Biol Sci, Ede, Osun State, Nigeria

[4] Irrua Specialist Teaching Hosp, Inst Lassa Fever Res & Control, Irrua, Edo State, Nigeria

[5] MIT, Broad Inst, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[6] Harvard Univ, Cambridge, MA 02138 USA

[7] Harvard Univ, Dept Organism & Evolutionary Biol, Ctr Syst Biol, Cambridge, MA 02138 USA

[8] Bernhard Nocht Inst Trop Med, Hamburg, Germany

[9] German Ctr Infect Res DZIF, Hamburg, Germany

[10] Harvard Univ, Dept Immunol & Infect Dis, Harvard TH Chan Sch Publ Hlth, Boston, MA 02115 USA

[11] Beth Israel Deaconess Med Ctr, Div Infect Dis, Boston, MA 02215 USA

[12] Eastern Polytech Inst, Kenema, Sierra Leone

[13] Kenema Govt Hosp, Viral Hemorrhag Fever Program, Kenema, Sierra Leone

[14] Minist Hlth & Sanitat, Freetown, Sierra Leone

[15] Irrua Specialist Teaching Hosp, Dept Med, Irrua, Edo State, Nigeria

[16] Ambrose Alli Univ, Fac Clin Sci, Dept Med, Ekpoma, Edo State, Nigeria

[17] Univ Sierra Leone, Coll Med & Allied Hlth Sci, Freetown, Sierra Leone

[18] Tulane Univ, Sch Med, Dept Pediat, Sect Infect Dis, New Orleans, LA 70112 USA

[19] Tulane Univ, Sch Med, Dept Internal Med, New Orleans, LA 70112 USA

[20] MIT, Harvard MIT Hlth Sci & Technol, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[21] Howard Hughes Med Inst, Chevy Chase, MD USA

[22] Tulane Univ, Tulane Hlth Sci Ctr, New Orleans, LA 70118 USA

[23] Tulane Univ, Sch Med, Dept Microbiol & Immunol, New Orleans, LA 70112 USA

来源：

SCIENTIFIC REPORTS | 2020年 / 10卷 / 01期

关键词：

SIERRA-LEONE; VIRUS; INFECTION; RNA; FILOVIRUSES; THERAPY; ASSAY;

D O I：

10.1038/s41598-020-65736-0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Lassa virus (LASV) is the causative agent of Lassa fever (LF), an often-fatal hemorrhagic disease. LF is endemic in Nigeria, Sierra Leone and other West African countries. Diagnosis of LASV infection is challenged by the genetic diversity of the virus, which is greatest in Nigeria. The ReLASV Pan-Lassa Antigen Rapid Test (Pan-Lassa RDT) is a point-of-care, in vitro diagnostic test that utilizes a mixture of polyclonal antibodies raised against recombinant nucleoproteins of representative strains from the three most prevalent LASV lineages (II, III and IV). We compared the performance of the Pan-LASV RDT to available quantitative PCR (qPCR) assays during the 2018 LF outbreak in Nigeria. For patients with acute LF (RDT positive, IgG/IgM negative) during initial screening, RDT performance was 83.3% sensitivity and 92.8% specificity when compared to composite results of two qPCR assays. 100% of samples that gave Ct values below 22 on both qPCR assays were positive on the Pan-Lassa RDT. There were significantly elevated case fatality rates and elevated liver transaminase levels in subjects whose samples were RDT positive compared to RDT negative.

引用

页数：14

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