Selective recognition of G-quadruplex in the vascular endothelial growth factor gene with small-molecule natural products by electrospray ionization (ESI) mass spectrometry and circular dichroism (CD) spectrometry

被引：4
|
作者：
He, Xiangwei [1 ]
Zhang, Zhenjiang [1 ]
Zhang, Qiang [1 ]
Yuan, Gu [1 ]
机构：
[1] Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Dept Chem Biol,Coll Chem, Beijing 100871, Peoples R China
来源：
CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE | 2012年 / 90卷 / 01期
基金：
中国国家自然科学基金;
关键词：
VEGF gene; natural products; DNA recognition; ESI mass spectrometry; CD spectroscopy; PROXIMAL PROMOTER REGION; INTERACTIVE AGENTS; TUMOR-CELLS; DNA; BINDING; IDENTIFICATION; DERIVATIVES; AFFINITY; LIGAND; MOTIF;
D O I：
10.1139/V11-104
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
In this study, electrospray ionization mass spectrometry (ESI-MS) and circular dichroism (CD) spectroscopy were used to investigate selective recognition of G-quadruplex in the vascular endothelial growth factor (VEGF) gene with 12 small-molecule natural products. We found that kaempferol, a natural flavonol, shows the highest binding affinity among the 12 natural molecules. The results from ESI-MS and CD spectra indicated that kaempferol could enhance the thermal stability of the VEGF G-quadruplex and showed selective recognition for the G-quadruplex in a solution consisting of the G-quadruplex and the corresponding duplex DNA.
引用
收藏
页码：55 / 59
页数：5
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