Regulation of vocal fold transepithelial water fluxes

被引:45
|
作者
Fisher, KV
Telser, A
Phillips, JE
Yeates, DB
机构
[1] Northwestern Univ, Dept Commun Sci & Disorders, Evanston, IL 60208 USA
[2] Univ Illinois, Dept Med, Chicago, IL 60612 USA
[3] Northwestern Univ, Dept Cell & Mol Biol, Evanston, IL 60208 USA
[4] Vet Affairs Chicago Hlth Care Syst, Chicago, IL 60612 USA
关键词
ion transport; sodium-potassium pump; larynx; voice; dog;
D O I
10.1152/jappl.2001.91.3.1401
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Vocal fold hydration is critical to phonation. We hypothesized that the vocal fold generates bidirectional water fluxes, which are regulated by activity of the Na+-K+ ATPase. Western blots and immunohistochemistry demonstrated the presence of the alpha -subunit Na+-K+ -ATPase in the canine vocal fold (n = 11). Luminal cells, basal and adjacent one to two layers of suprabasal cells within stratified squamous epithelium, were immunopositive, as well as basolateral membranes of submucosal seromucous glands underlying transitional epithelia. Canine (n = 6) and ovine (n = 14) vocal fold mucosae exhibited transepithelial potential differences of 8.1 +/-2.8 and 9.3 +/-1.3 mV (lumen negative), respectively. The potential difference and short-circuit current (ovine = 31 +/-4 muA/cm(2); canine = 41 +/- 10 muA/cm(2)) were substantially reduced by luminal administration of 75 muM acetylstrophanthidin (P<0.05). Ovine (n = 7) transepithelial water fluxes decreased from 5.1<plus/minus>0.3 to 4.3 +/-0.3 mul.min(-1).cm(-2) from the basal to luminal chamber and from 5.2 +/-0.2 to 3.9 +/-0.3 mul.min(-1).cm(-2) from the luminal to basal chamber by luminal acetylstrophanthidin (P<0.05). The presence of the Na+-K+ -ATPase in the vocal fold epithelium and the electrolyte transport derived from its activity provide the intrinsic mechanisms to regulate cell volume as well as vocal fold hydration.
引用
收藏
页码:1401 / 1411
页数:11
相关论文
共 50 条
  • [1] The Effects of Cigarette Smoke Condensate on Vocal Fold Transepithelial Resistance and Inflammatory Signaling in Vocal Fold Fibroblasts
    Branski, Ryan C.
    Zhou, Hang
    Kraus, Dennis H.
    Sivasankar, Mahalakshmi
    [J]. LARYNGOSCOPE, 2011, 121 (03): : 601 - 605
  • [2] Vasopressin increases airway transepithelial water fluxes.
    Phillips, JE
    Yeates, DB
    [J]. FASEB JOURNAL, 1999, 13 (04): : A397 - A397
  • [3] Shear Stress, Intracellular Calcium, and Transepithelial Water Fluxes
    Warren, N. J.
    Crampin, E. J.
    Tawhai, M. H.
    [J]. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2009, 179
  • [4] Evidence for cAMP-mediated transepithelial ion flux in porcine vocal fold epithelia
    Sivasankar, Mahalakshmi
    Nofziger, Charity
    Blazer-Yost, Bonnie
    [J]. FASEB JOURNAL, 2008, 22
  • [5] Mechanical Regulation of Human Vocal Fold Stellate Cells
    Sato, Kiminori
    Kurita, Takashi
    Chitose, Shun-ichi
    Umeno, Hirohito
    Nakashima, Tadashi
    [J]. ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY, 2015, 124 (01): : 49 - 54
  • [6] Regulation of aquaporin 1 in epithelia of vocal fold and trachea
    Lodewyck, D
    Jilling, T
    Menco, B
    Fisher, K
    [J]. FASEB JOURNAL, 2006, 20 (04): : A350 - A350
  • [7] Vocal fold paresis accompanying vocal fold polyps
    Sevtap Akbulut
    Rahsan Adviye Inan
    Hande Altintas
    Ibrahim Gul
    Derya Berk
    Mustafa Paksoy
    [J]. European Archives of Oto-Rhino-Laryngology, 2015, 272 : 149 - 157
  • [8] Vocal fold paresis accompanying vocal fold polyps
    Akbulut, Sevtap
    Inan, Rahsan Adviye
    Altintas, Hande
    Gul, Ibrahim
    Berk, Derya
    Paksoy, Mustafa
    [J]. EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY, 2015, 272 (01) : 149 - 157
  • [9] Transepithelial SCFA fluxes link intracellular and extracellular pH regulation of mouse colonocytes
    Chu, SY
    Montrose, MH
    [J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 1997, 118 (02): : 403 - 405
  • [10] Suture laterofixation of the vocal fold for bilateral vocal fold immobility
    Damrose, Edward J.
    [J]. CURRENT OPINION IN OTOLARYNGOLOGY & HEAD AND NECK SURGERY, 2011, 19 (06): : 416 - 421