Template-Free In Situ Encapsulation of Enzymes in Hollow Covalent Organic Framework Capsules for the Electrochemical Analysis of Biomarkers

被引:48
|
作者
Chao, Hao [1 ]
Zhou, Zixin [1 ]
He, Wenting [1 ]
Li, Meng [1 ]
Yuan, Xiaoyu [1 ]
Su, Ping [1 ]
Song, Jiayi [1 ]
Yang, Yi [1 ]
机构
[1] Beijing Univ Chem Technol, Coll Chem, Beijing Key Lab Environm Harmful Chem Anal, Beijing 100029, Peoples R China
基金
中国国家自然科学基金; 北京市自然科学基金;
关键词
template-free method; in situ; enzyme encapsulation; covalent organic frameworks; capsules; EMBEDDING ENZYMES; IMMOBILIZATION; STABILITY;
D O I
10.1021/acsami.2c01357
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Although capsule-like materials as host carriers for enzyme encapsulation have been a hot topic in recent years, creating an ideal microenvironment for enhanced enzymatic performance is still a formidable challenge. Herein, we created a template-free method to in situ encapsulate natural enzymes in hollow covalent organic framework (COF) capsules at room temperature. The COF crystallites migrated from the inner core and self-assembled at the outside walls during the inside-out Ostwald ripening process, retaining the enzymes in the cavity. The adjustable hollow structure of the enzyme@COF capsule allowed the basic vibration of the enzyme to maintain a certain degree of freedom, thus significantly enhancing the enzymatic bioactivity. The hollow enzyrne@COF capsule has large mesoporous tunnels allowing the efficient transport. In addition, the enzyme encapsulated in the capsule showed superior activity and ultrahigh stability under various extreme conditions that may lead to enzyme inactivation, such as high temperature, organic solvents, chelates, and the denaturing agent. Finally, the prepared hollow GOx@COF capsule was used for electrochemical sensing of glucose in human serum, and the electrochemical sensor exhibited high selectivity and satisfactory test results. This research not only provides a new way for COFs to encapsulate enzymes but also has potential applications in biocatalysis and biosensing, making artificial organelles possible.
引用
收藏
页码:20641 / 20651
页数:11
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