Chromatographic removal of endotoxin from hemoglobin preparations - Effects of solution conditions on endotoxin removal efficiency and protein recovery

In this work, affinity chromatography was used to remove endotoxin from human hemoglobin preparations with a Sterogene Acticlean Etox column. The effects of solution conditions on endotoxin removal efficiency and protein recovery have been investigated. It has been found that cations Na+ or Ca+ reduced endotoxin removal efficiency from 73% (sample prepared with endotoxin-free water) to 31% (sample prepared with 0.15 M NaCl, ionic strength, I=0.15 M), and from 73% (sample prepared with endotoxin-free water) to 9% (sample prepared with 0.05 M CaCl2, ionic strength, I=0.15 M). It has also been found that the protein recovery was increased from 90% to 96%, and from 90% to 99%, respectively. (C) 1998 Elsevier Science B.V. All rights reserved.