Phytochemical and biological evaluation of metabolites produced by alginate-immobilized Bionts isolated from the lichen Cladonia substellata vain

被引：6

作者：

Santiago, Rocio ^{[1
]}

Martins, Monica C. B. ^{[1
]}

Vilaca, Matheus D. ^{[1
]}

de Barros, Luiz F. B. ^{[2
]}

Nascimento, Tais ^{[2
]}

da Silva, Nicacio H. ^{[2
]}

Falcao, Emerson P. S. ^{[3
]}

Legaz, Maria-Estrella ^{[4
]}

Vicente, Carlos ^{[4
]}

Pereira, Eugenia C. ^{[5
]}

机构：

[1] Univ Fed Pernambuco, Postgrad Program Plant Biol, Recife, PE, Brazil

[2] Univ Fed Pernambuco, Biochem Dept, Recife, PE, Brazil

[3] Univ Fed Pernambuco, Vitoria Acad Ctr, Lab Synth & Mol Isolat, Recife, PE, Brazil

[4] Univ Complutense Madrid, Team Cell Interact Plant Symbiosis, Madrid, Spain

[5] Univ Fed Pernambuco, Philosophy & Human Sci Ctr, Dept Geog Sci, Recife, PE, Brazil

来源：

FITOTERAPIA | 2018年 / 131卷

关键词：

Alginate; Antimicrobial activity; Antioxidant activity; Cladoniaceae; Immobilization; Isolated symbionts; Lichen substances; ANTIOXIDANT ACTIVITY; USNIC ACID; ANTIMICROBIAL ACTIVITY; SECONDARY METABOLITES; PARMELIA-CAPERATA; MYCOBIONTS; RESYNTHESIS; SUBSTANCES; MECHANISMS; GROWTH;

D O I：

10.1016/j.fitote.2018.10.001

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

In this work, new biotechnological procedures have been optimized on the basis of immobilization in alginate of bionts isolated from the lichen C. substellata. From these immobilizates, soluble and biologically active phenolics can be obtained. During bionts-immobilization, stictic, norstictic and usnic acids were secreted to the medium. The amount produced of each of them differed depending on the immobilization time, the precursor supplied and the type of biont used. Greater amounts of stictic acid were detected and maintained over time in all bioreactors. The opposite occurs in non-immobilized thallus. Virtually, all lichen phenols exhibit antioxidant activity to a greater or lesser degree, so that the antioxidant capacity of stictic acid (82.13% oxidation inhibition) was tested. The soluble extract of immobilized algae co-incubated in sodium acetate with fungal hyphae contained carbohydrates and exhibited a potent antioxidant capacity after 13 days of immobilization (94.87%). Therefore, attempts have been made to relate both parameters. On the other hand, the growth of Staphylococcus aureus, Escherichia coil and Klebsiella pnewnoniae was inhibited by phenolic compounds produced by immobilizates, although the organic extract of the whole lichen showed the highest activity due to a possible synergy with other indeterminate compounds. Thus, C. substellata immobilized bionts are a potential source of different natural antioxidant and antimicrobial compounds.

引用

页码：23 / 34

页数：12