Exo-arabinanase of Penicillium chrysogenum able to release arabinobiose from α-1,5-L-arabinan

被引：39
|
作者：
Sakamoto, T
Thibault, JF
机构：
[1] Inst Natl Rech Agron, Unite Rech Polysaccharides Org & Interact, F-44316 Nantes 3, France
[2] Univ Osaka Prefecture, Grad Sch Agr & Biol Sci, Div Appl Biochem, Osaka 5998531, Japan
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2001年 / 67卷 / 07期
关键词：
D O I：
10.1128/AEM.67.7.3319-3321.2001
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An exo-arabinanase, designated Abnx, was purified from a culture filtrate of Penicillium chrysogenum 31B by ammonium sulfate precipitation, anion-exchange chromatography, and hydrophobic chromatography. Abnx had an apparent molecular mass of 47 kDa. The enzyme released only arabinobiose from the nonreducing terminus of alpha -1,5-L-arabinan and shelved no activity towards p-nitrophenyl-alpha -L-arabinofuranoside and alpha -1,5-L-arabinofuranobiose. Abnx is the first enzyme with this mode of action.
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收藏
页码：3319 / 3321
页数：3
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