Nanodiamond as a Vector for siRNA Delivery to Ewing Sarcoma Cells

被引：179

作者：

Alhaddad, Anna ^{[1
,2
,3
]}

Adam, Marie-Pierre ^{[4
,5
]}

Botsoa, Jacques ^{[4
,5
]}

Dantelle, Geraldine ^{[6
,7
]}

Perruchas, Sandrine ^{[6
,7
]}

Gacoin, Thierry ^{[6
,7
]}

Mansuy, Christelle ^{[1
,8
,9
,10
]}

Lavielle, Solange ^{[8
,9
,10
]}

Malvy, Claude ^{[2
,3
]}

Treussart, Francois ^{[4
,5
]}

Bertrand, Jean-Remi ^{[1
,2
,3
]}

机构：

[1] Univ Paris 11, Lab Vectorol & Therapeut Anticancereuses, UMR 8203, F-91405 Orsay, France

[2] CNRS, Lab Vectorol & Therapeut Anticancereuses, UMR 8203, F-91405 Orsay, France

[3] Inst Gustave Roussy, Lab Vectorol & Therapeut Anticancereuses, UMR 8203, F-94805 Villejuif, France

[4] CNRS, Lab Photon Quant & Mol, F-94235 Cachan, France

[5] Ecole Normale Super, UMR 8537, F-94235 Cachan, France

[6] Ecole Polytech, UMR 7643, F-91120 Palaiseau, France

[7] CNRS, Lab Phys Mat Condensee, F-91120 Palaiseau, France

[8] Univ Paris 06, Lab BioMol, UMR 7203, F-75005 Paris, France

[9] Ecole Normale Super, Dept Chim, Lab BioMol, UMR 7203, F-75231 Paris, France

[10] CNRS, Lab BioMol, UMR 7203, F-75231 Paris, France

来源：

SMALL | 2011年 / 7卷 / 21期

关键词：

SMALL INTERFERING RNA; IN-VIVO; FLUORESCENT NANODIAMONDS; JUNCTION ONCOGENE; ANTISENSE OLIGONUCLEOTIDES; DRUG-DELIVERY; NANOPARTICLES; BIODISTRIBUTION; NANOTECHNOLOGIES; EXPRESSION;

D O I：

10.1002/smll.201101193

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The ability of diamond nanoparticles (nanodiamonds, NDs) to deliver small interfering RNA (siRNA) into Ewing sarcoma cells is investigated with a view to the possibility of in-vivo anticancer nucleic-acid drug delivery. siRNA is adsorbed onto NDs that are coated with cationic polymer. Cell uptake of NDs is demonstrated by taking advantage of the NDs' intrinsic fluorescence from embedded color-center defects. Cell toxicity of these coated NDs is shown to be low. Consistent with the internalization efficacy, a specific inhibition of EWS/Fli-1 gene expression is shown at the mRNA and protein level by the ND-vectorized siRNA in a serum-containing medium.

引用

页码：3087 / 3095

页数：9

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