Regulation of neuronal growth cone filopodia by nitric oxide

Nitric oxide (NO) has been proposed to play an important role during neuronal development. Since many of its effects occur during the time of growth cone pathfinding and target interaction, ne here test the hypothesis that part of NO's effects might be exerted at the growth cone. Ne found that los concentrations of the NO-donors DEA/NO, SIN-1, and SNP caused a rapid and transient elongation of filopodia as well as a reduction in filopodial number. These effects resulted from distinct changes in filopodial extension and retraction rates. Our novel findings suggest that SO could play a physiological role by temporary changing a growth cone's morphology and switching its behavior from a close-range to a long-range exploratory mode. We subsequently dissected the pathway by which NO acted on growth cones, The effect of SO donors on filopodial length could be blocked by 1H-[1,2,4]oxadiazolo[4,3- a]quinosalin-1-one. an inhibitor of soluble guanylyl cyclase (sGC), indicating that KO acted via sGC. Supporting this idea, injection of cyclic GMP (cGMP) mimicked the effect of NO donors on growth cone filopodia, Moreover, application of SO-donors as Hell as injection of cGMP elicited a rapid and transient rise in intracellular calcium in growth cones, indicating that SO acted via cGMP to elevate calcium. This calcium rise, as well as the morphological effects of SIN-1 on filopodia, were blocked by preventing calcium entry. Given the role of filopodia in axonal guidance, our new data suggest that NO could function at the neuronal growth cone as an intracellular and/or intercellular signaling molecule by affecting steering decisions during neuronal pathfinding. (C) 1999 John Wiley & Sans. Inc.