Knockdown of ezrin suppresses the migration and angiogenesis of human umbilical vein endothelial cells in vitro

被引:7
|
作者
Zhao, Liang-ping [1 ]
Huang, Lei [1 ]
Tian, Xun [1 ]
Liang, Feng-qi [1 ]
Wei, Jun-cheng [2 ]
Zhang, Xian [1 ]
Li, Sha [1 ]
Zhang, Qing-hua [1 ]
机构
[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Wuhan Cent Hosp, Dept Gynecol & Obstet, Wuhan 430014, Peoples R China
[2] Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Dept Gynecol & Obstet, Wuhan 430030, Peoples R China
基金
中国国家自然科学基金;
关键词
ezrin; RNA interference; human umbilical vein endothelial cell; migration; angiogenesis; TUBE FORMATION; ERM PROTEINS; MECHANISMS; MOLECULES; FAMILY;
D O I
10.1007/s11596-016-1574-y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Progressive tumor growth is dependent on angiogenesis. The mechanisms by which endothelial cells (ECs) are incorporated to develop new blood vessels are not well understood. Recent studies reveal that the ezrin radixin moesin (ERM) family members are key regulators of cellular activities such as adhesion, morphogenetic change, and migration. We hypothesized that ezrin, one of the ERM family members, may play important roles in ECs organization during angiogenesis, and new vessels formation in preexisting tissues. To test this hypothesis, in this study, we investigated the effects of ezrin gene silencing on the migration and angiogenesis of human umbilical vein endothelial cells (HUVECs) in vitro. HUVECs were transfected with plasmids with ezrin-targeting short hairpin RNA by using the lipofectamine-2000 system. Wound assay in vitro and three-dimensional culture were used to detect the migration and angiogenesis capacity of HUVECs. The morphological changes of transfected cells were observed by confocal and phase contrast microscopy. Our results demonstrated that the decreased expression of ezrin in HUVECs significantly induced the morphogenetic changes and cytoskeletal reorganization of the transfected cells, and also reduced cell migration and angiogenesis capacity in vitro, suggesting that ezrin play an important role in the process of HUVECs migration and angiogenesis.
引用
收藏
页码:243 / 248
页数:6
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