The biometrical studies of the 24 buffalo livers (12 male and 12 female) were carried out. The average weight of liver of male and female buffalo was 5949 +/- 340.10 gm and 6358.33 +/- 243.23 gm, respectively. The average volume of liver of male and female buffalo was 5075+277.95 cc and 5350 +/- 212.67 cc, respectively. The average density of liver of male and female buffalo liver was 1.16+0.02 gm/cc and 1.19+0.02 gm/cc, respectively. The average curvature length on parietal surface of liver of male and female buffalo liver was 54.77+1.33 cm and 55.25+1.38 cm, respectively. The average width at falciform ligament of liver of male and female buffalo liver was 16.42+0.62 cm and 17.54+0.47 cm, respectively. The average curvature width on the middle of parietal surface of liver of male and female buffalo was 28.08+0.89 cm and 29.17+0.51 cm, respectively. The average curved length on visceral surface from portal vein to apex of liver of male and female buffalo liver was 23.24+2.09 cm and 20.95+1.11 cm, respectively. The average maximum length of caudate lobe of liver of male and female buffalo liver was 11.52+0.94 cm and 11.88+0.50 cm, respectively. The average maximum width of caudate lobe on visceral surface of liver of male and female of buffalo liver was 7.90+0.27 cm and 7.90+0.20 cm respectively. The average curvature width on visceral surface from umbilical fissure to esophageal notch of liver of male and female buffalo liver was 21.013+0.39 cm and 21.11+0.34 cm, respectively. The average curvature width on the middle of visceral surface of liver of male and female buffalo liver was 30.63+0.76 cm and 31.56+0.50 cm, respectively. The average curvature length from umbilical fissure to portal vein of liver of male and female buffalo liver was 20.55+0.78 cm and 21.51+0.25 cm, respectively. The average curvature length from portal length to esophageal notch of liver of male and female buffalo liver was 20.51+1.09 cm and 19.86+8.83 cm, respectively. The average curvature length from esophageal notch to umbilical fissure of liver of male and female buffalo liver was 19.55+0.66 cm and 19.95+0.66 cm, respectively. The average curved length of ridge separating the omasal and reticular impression of liver of male and female buffalo liver was 29.33+1.12 cm and 31.01+1.48 cm, respectively. The average curvature length from esophageal notch to tip of main lobe of liver of male and female buffalo liver was 37.50+0.92 cm and 37.37+0.95 cm, respectively. The average length from tip of main lobe to tip caudate lobe of liver of male and female buffalo liver was 7.16+0.34 cm and 6.63+0.20 cm, respectively. The average curvature length from caudate length from caudate lobe to umbilical fissure of liver of male and female buffalo liver was 30.03+1.26 cm and 28.30+1.49 cm, respectively.