Crystallization and preliminary crystallographic analysis of the pyruvate-ferredoxin oxidoreductase from Desulfovibrio africanus

被引：7
|
作者：
Pieulle, L
Chabrière, E
Hatchikian, C
Fontecilla-Camps, JC
Charon, MH
机构：
[1] CNRS, Unite Bioenerget & Ingn Proteines, F-13402 Marseille 20, France
[2] CEA, CNRS, Inst Biol Struct J P Ebel, Cristallog & Cristallogenese Prot Lab, F-38027 Grenoble, France
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1999年 / 55卷
关键词：
D O I：
10.1107/S0907444998008920
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
For the first time, crystals of a pyruvate-ferredoxin oxidoreductase (PFOR) suitable for X-ray analysis have been obtained. This enzyme catalyzes, in anaerobic organisms, the crucial energy-yielding reaction of pyruvate decarboxylation to acetylCoA. Polyethylene glycol and divalent metal cations have been used to crystallize the PFOR from the sulfate-reducing bacterium Desulfovibrio africanus. Two different orthorhombic (P2(1)2(1)2(1)) crystal forms have been grown with unit-cell dimensions a = 86.1, b = 146.7, c = 212.5 Angstrom and a = 84.8, b = 144.9, c = 203.0 Angstrom, Both crystals diffract to 2.3 Angstrom resolution using synchrotron radiation.
引用
收藏
页码：329 / 331
页数：3
相关论文
共 50 条
  • [1] Structural and kinetic studies of the pyruvate-ferredoxin oxidoreductase/ferredoxin complex from Desulfovibrio africanus
    Pieulle, L
    Charon, MH
    Bianco, P
    Bonicel, J
    Pétillot, Y
    Hatchikian, EC
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 264 (02): : 500 - 508
  • [2] Octomeric pyruvate-ferredoxin oxidoreductase from Desulfovibrio vulgaris
    Garczarek, Florian
    Dong, Ming
    Typke, Dieter
    Witkowska, H. Ewa
    Hazen, Terry C.
    Nogales, Eva
    Biggin, Mark D.
    Glaeser, Robert M.
    [J]. JOURNAL OF STRUCTURAL BIOLOGY, 2007, 159 (01) : 9 - 18
  • [3] ISOLATION AND CHARACTERIZATION OF THE PYRUVATE-FERREDOXIN OXIDOREDUCTASE FROM THE SULFATE-REDUCING BACTERIUM DESULFOVIBRIO-AFRICANUS
    PIEULLE, L
    GUIGLIARELLI, B
    ASSO, M
    DOLE, F
    BERNADAC, A
    HATCHIKIAN, EC
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1995, 1250 (01): : 49 - 59
  • [4] PYRUVATE-FERREDOXIN OXIDOREDUCTASE IN CAMPYLOBACTER SPECIES
    DAUCHER, JA
    KRIEG, NR
    [J]. CANADIAN JOURNAL OF MICROBIOLOGY, 1995, 41 (02) : 198 - 201
  • [5] Flexibility of thiamine diphosphate revealed by kinetic crystallographic studies of the reaction of pyruvate-ferredoxin oxidoreductase with pyruvate
    Cavazza, C
    Contreras-Martel, C
    Pieulle, L
    Chabrière, E
    Hatchikian, EC
    Fontecilla-Camps, JC
    [J]. STRUCTURE, 2006, 14 (02) : 217 - 224
  • [6] IMPROVED PURIFICATION, CRYSTALLIZATION AND PRIMARY STRUCTURE OF PYRUVATE-FERREDOXIN OXIDOREDUCTASE FROM HALOBACTERIUM-HALOBIUM
    PLAGA, W
    LOTTSPEICH, F
    OESTERHELT, D
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 205 (01): : 391 - 397
  • [7] INACTIVATION OF CLOSTRIDIAL FERREDOXIN AND PYRUVATE-FERREDOXIN OXIDOREDUCTASE BY SODIUM-NITRITE
    CARPENTER, CE
    REDDY, DSA
    CORNFORTH, DP
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (03) : 549 - 552
  • [8] Isolation and analysis of the gene encoding the pyruvate-ferredoxin oxidoreductase of Desulfovibrio africanus, production of the recombinant enzyme in Escherichia coli, and effect of carboxy-terminal deletions on its stability
    Pieulle, L
    Magro, V
    Hatchikian, EC
    [J]. JOURNAL OF BACTERIOLOGY, 1997, 179 (18) : 5684 - 5692
  • [9] PURIFICATION AND CHARACTERIZATION OF THE PYRUVATE-FERREDOXIN OXIDOREDUCTASE FROM CLOSTRIDIUM-ACETOBUTYLICUM
    MEINECKE, B
    BERTRAM, J
    GOTTSCHALK, G
    [J]. ARCHIVES OF MICROBIOLOGY, 1989, 152 (03) : 244 - 250
  • [10] PYRUVATE-FERREDOXIN OXIDOREDUCTASE .4. STUDIES ON REACTION MECHANISM
    UYEDA, K
    RABINOWITZ, JC
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1971, 246 (10) : 3120 - +
12345