Per oral rat treatment with glyconjugate fractions of Genipa americana leaves protects thrombus formation

The current study evaluated the effect of the arabinogalactan-glycoconjugate fractions (FI and FII) isolated from Genipa americana leaves given per oral in rat hemostasis protocols. Rats received daily treatment with FI or FII during 7 days and were evaluated for coagulation, platelet aggregation, venous thrombosis and bleeding tendency 1 h after the last treatment. FII prolonged in 5.5-fold the rat plasma coagulation time (activated partial thromboplastin time test). FI inhibited by 46% the platelet aggregation. Both FI and FII prevented thrombus formation by 33 and 28%, respectively. However, the bleeding time was not altered by any fractions, showing an advantage in relation to acetylsalicylic acid or warfarin that increased the bleeding time in 3.6 and 2.9-fold, respectively. Per oral treatment with the arabinogalactan-glyconjugate fractions FI and FII of G. americana leaves in rats prevents thrombus formation, being devoid of hemorrhagic risk. These results bring novel therapeutic possibilities for thromboembolic diseases.