Evaluation of Antioxidative Mechanisms In Vitro and Triterpenes Composition of Extracts from Silver Birch (Betula pendula Roth) and Black Birch (Betula obscura Kotula) Barks by FT-IR and HPLC-PDA

被引：12

作者：

Ostapiuk, Aleksandra ^{[1
]}

Kurach, Lukasz ^{[2
]}

Strzemski, Maciej ^{[3
]}

Kurzepa, Jacek ^{[1
]}

Hordyjewska, Anna ^{[1
]}

机构：

[1] Med Univ Lublin, Dept Med Chem, 4A Chodzki Str, PL-20093 Lublin, Poland

[2] Med Univ Lublin, Independent Lab Behav Studies, 4A Chodzki Str, PL-20093 Lublin, Poland

[3] Med Univ Lublin, Dept Analyt Chem, 4A Chodzki Str, PL-20093 Lublin, Poland

来源：

MOLECULES | 2021年 / 26卷 / 15期

关键词：

Betula pendula Roth; Betula obscura Kotula; silver birch; black birch; pentacyclic triterpenes; CELL-DEATH; ACID; VIVO; RAMAN;

D O I：

10.3390/molecules26154633

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Silver birch, Betula pendula Roth, is one of the most common trees in Europe. Due to its content of many biologically active substances, it has long been used in medicine and cosmetics, unlike the rare black birch, Betula obscura Kotula. The aim of the study was therefore to compare the antioxidant properties of extracts from the inner and outer bark layers of both birch trees towards the L929 line treated with acetaldehyde. Based on the lactate dehydrogenase test and the MTT test, 10 and 25% concentrations of extracts were selected for the antioxidant evaluation. All extracts at tested concentrations reduced the production of hydrogen peroxide, superoxide anion radical, and 25% extract decreased malonic aldehyde formation in acetaldehyde-treated cells. The chemical composition of bark extracts was accessed by IR and HPLC-PDA methods and surprisingly, revealed a high content of betulin and lupeol in the inner bark extract of B. obscura. Furthermore, IR analysis revealed differences in the chemical composition of the outer bark between black and silver birch extracts, indicating that black birch may be a valuable source of numerous biologically active substances. Further experiments are required to evaluate their potential against neuroinflammation, cancer, viral infections, as well as their usefulness in cosmetology.

引用

页数：15