Thirteen phenolic compounds from Aloe barbadensis (syn. A. vera) and A. arborescens were analysed by high performance liquid chromatography, using a reverse phase column eluted with a methanol: water gradient, and detected by UV at 293 nm. Aloesin, 8-C-glucosyI-7-O-methyl-(S)-aloesol, neoaloesin A, 8-O-methyl-7-hydroxyaloin A and B, 10-hydroxyaloin A, isoaloeresin D, aloin A and B, aloeresin E and aloe-emodin from A. barbadensis: and aloenin, aloenin B, 10-hydroxyaloin A, aloin A and B, and aloe-emodin from A. arborescens, were identified and quantified. The seasonal variation of two Aloe species was also examined by this method. (C) 1998 John Wiley & Sons, Ltd.