The flagellin hypervariable region is a potential flagella display domain in probiotic Escherichia coli strain Nissle 1917

被引：21
|
作者：
Yang, Ying [1 ,2 ]
Yang, Yi [1 ,2 ]
Ou, Bingming [1 ,2 ]
Xia, Pengpeng [1 ,2 ]
Zhou, Mingxu [1 ,2 ]
Li, Luan [1 ,2 ,3 ]
Zhu, Guoqiang [1 ,2 ]
机构：
[1] Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Jiangsu, Peoples R China
[2] Jiangsu Coinnovat Ctr Important Anim Infect Dis &, Yangzhou 225009, Jiangsu, Peoples R China
[3] Univ British Columbia, Fac Land & Food Syst, Vancouver, BC, Canada
来源：
ARCHIVES OF MICROBIOLOGY | 2016年 / 198卷 / 07期
基金：
美国国家科学基金会;
关键词：
E. coli strain Nissle 1917; Flagella display application; Flagellin; SALMONELLA FLAGELLIN; ORAL INFECTION; CELL-LINES; EXPRESSION; COLONIZATION; CONTRIBUTES; INHIBITOR; INTESTINE; VIRULENCE; MOTILITY;
D O I：
10.1007/s00203-016-1219-3
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The most studied probiotic, Escherichia coli strain Nissle 1917 (EcN) possesses flagella of serotype H1. To explore the potential to use EcN flagellin in flagella display applications, we investigated the effect of deleting amino acids in the hypervariable region of flagellin on EcNc (EcN cured of its two cryptic plasmids pMUT1 and pMUT2). Two EcNc flagellin isogenic mutants with deletions of amino acid residual from 277 to 286 and from 287 to 296 in the hypervariable domain were constructed. Both mutants were flagellated, adherent to IPEC-J2 cells, and colonized BALB/c mice. These hypervariable regions may have future utility in the display of heterologous epitopes.
引用
收藏
页码：603 / 610
页数：8
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