Label-free coherent anti-stokes Raman scattering imaging of coexisting lipid domains in single bilayers

被引：21
|
作者：
Li, Li [1 ,2 ]
Cheng, Ji-Xin [1 ,2 ]
机构：
[1] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA
[2] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA
来源：
JOURNAL OF PHYSICAL CHEMISTRY B | 2008年 / 112卷 / 06期
关键词：
D O I：
10.1021/jp711527v
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Laser-scanning coherent anti-Stokes Raman scattering (CARS) microscopy was used to image lipid domains in single bilayers without any labeling. On the basis of the molecular packing density difference between liquid-disordered (L-d) liquid-ordered (L-o), and gel (S-o) phases, clear vibrational contrasts were generated between coexisting domains in a single bilayer of DOPC/DPPC (1:1) and DOPC/DPPC/cholesterol (4:4:2). The method reported here can be potentially applied to study phase segregation in live cell membranes which are highly heterogeneous and dynamic.
引用
收藏
页码：1576 / 1579
页数：4
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