Infection with CagA-positive Helicobacter pylori strain containing three EPIYA C phosphorylation sites is associated with more severe gastric lesions in experimentally infected Mongolian gerbils (Meriones unguiculatus)

被引：20

作者：

Ferreira Junior, M. ^{[1
]}

Batista, S. A. ^{[2
]}

Vidigal, P. V. T. ^{[3
]}

Cordeiro, A. A. C. ^{[1
]}

Oliveira, F. M. S. ^{[1
]}

Prata, L. O. ^{[1
]}

Diniz, A. E. T. ^{[4
]}

Barral, C. M. ^{[4
]}

Barbuto, R. C. ^{[4
]}

Gomes, A. D. ^{[2
]}

Araujo, I. D. ^{[4
]}

Queiroz, D. M. M. ^{[2
]}

Caliari, M. V. ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Inst Biol Sci, Dept Gen Pathol, BR-31270010 Belo Horizonte, MG, Brazil

[2] Univ Fed Minas Gerais, Fac Med, Lab Res Bacteriol, BR-31270010 Belo Horizonte, MG, Brazil

[3] Univ Fed Minas Gerais, Fac Med, Dept Anat Pathol, BR-31270010 Belo Horizonte, MG, Brazil

[4] Univ Fed Minas Gerais, Fac Med, Dept Surg, BR-31270010 Belo Horizonte, MG, Brazil

来源：

EUROPEAN JOURNAL OF HISTOCHEMISTRY | 2015年 / 59卷 / 02期

关键词：

Gastritis; atrophy; intestinal metaplasia; dysplasia; Mongolian gerbil; cagA EPIYA C motif; PATHOGENICITY ISLAND; RISK; RECOMBINATION; INFLAMMATION; BACTERIA; CANCER; ULCER; CELLS; MODEL; HOST;

D O I：

10.4081/ejh.2015.2489

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Infection with Helicobacter pylori strains containing high number of EPIYA-C phosphorylation sites in the CagA is associated with significant gastritis and increased risk of developing pre-malignant gastric lesions and gastric carcinoma. However, these findings have not been reproduced in animal models yet. Therefore, we investigated the effect on the gastric mucosa of Mongolian gerbil (Meriones unguiculatus) infected with CagA-positive H. pylori strains exhibiting one or three EPIYA-C phosphorilation sites. Mongolian gerbils were inoculated with H. pylori clonal isolates containing one or three EPIYA-C phosphorylation sites. Control group was composed by uninfected animals challenged with Brucella broth alone. Gastric fragments were evaluated by the modified Sydney System and digital morphometry. Clonal relatedness between the isolates was considered by the identical RAPD-PCR profiles and sequencing of five housekeeping genes, vacA i/d region and of oipA. The other virulence markers were present in both isolates (vacA s1i1d1m1, iceA2, and intact dupA). CagA of both isolates was translocated and phosphorylated in AGS cells. After 45 days of infection, there was a significant increase in the number of inflammatory cells and in the area of the lamina propria in the infected animals, notably in those infected by the CagA-positive strain with three EPIYA-C phosphorylation sites. After six months of infection, a high number of EPIYA-C phosphorylation sites was associated with progressive increase in the intensity of gastritis and in the area of the lamina propria. Atrophy, intestinal metaplasia, and dysplasia were also observed more frequently in animals infected with the CagA-positive isolate with three EPIYA-C sites. We conclude that infection with H. pylori strain carrying a high number of CagA EPIYA-C phosphorylation sites is associated with more severe gastric lesions in an animal model of H. pylori infection. Non

引用

页码：137 / 146

页数：10

共 1 条

[1] CagA-positive Helicobacter pylori strain containing three EPIYA C phosphorylation sites produces increase of G cell and decrease of D cell in experimentally infected gerbils (Meriones unguiculatus)
Ferreira Junior, Moacir
Batista, Sergio de Assis
Barbuto, Rafael Calvao
Gomes, Adriana Dias
Magalhaes Queiroz, Dulciene Maria
Araujo, Ivana Duval
Caliari, Marcelo Vidigal
ADVANCES IN MEDICAL SCIENCES, 2016, 61 (02): : 231 - 236

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