Improvement of catalytic activity of lipase from Candida rugosa via sol-gel encapsulation in the presence of calix(aza)crown

被引：47
|
作者：
Uyanik, Arzu [1 ]
Sen, Nejdet [1 ]
Yilmaz, Mustafa [1 ]
机构：
[1] Selcuk Univ, Dept Chem, TR-42075 Konya, Turkey
来源：
BIORESOURCE TECHNOLOGY | 2011年 / 102卷 / 06期
关键词：
Candida rugosa lipase; Enantioselective hydrolysis; Sol-gel encapsulation; Calix(aza)crowns; Naproxen; ENANTIOSELECTIVE HYDROLYSIS; RACEMIC NAPROXEN; IMMOBILIZATION; MODULATION; RESOLUTION; ESTER;
D O I：
10.1016/j.biortech.2010.12.105
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
Lipase from Candida rugosa (CRL) was encapsulated within a chemically inert sol-gel support in the presence of calix(aza)crowns as the new additives. The catalytic activity of the encapsulated lipases was evaluated both in the hydrolysis of p-nitrophenyl palmitate (p-NPP) and the enantioselective hydrolysis of racemic Naproxen methyl ester. It has been observed that the percent activity yields of the calix(aza)crown based encapsulated lipases were higher than that of the free lipase. Improved enantioselectivity was observed with the calix(aza)crown-based encapsulated lipases as compared to encapsulated free lipase. The reaction of Naproxen methyl ester resulted in 48.4% conversion for 24 h and 98% enantiomeric excess for the S-acid, corresponding to an E value of > 300 (E = 166 for the encapsulated free enzyme). Moreover, the encapsulated lipases were still retained about 18% of their conversion ratios after the sixth reuse in the enantioselective reaction. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：4313 / 4318
页数：6
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    [J]. ORGANIC & BIOMOLECULAR CHEMISTRY, 2011, 9 (11) : 4021 - 4024
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    [J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2013, 170 (08) : 1871 - 1884
  • [3] Preparation of New Calix[4]arene-Immobilized Biopolymers for Enhancing Catalytic Properties of Candida rugosa Lipase by Sol–Gel Encapsulation
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  • [4] Improvement of catalytic properties of lipase from Arthrobacter sp by encapsulation in hydrophobic sol-gel materials
    Yang, Guang
    Wu, Jianping
    Xu, Gang
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    [J]. BIOORGANIC CHEMISTRY, 2016, 65 : 1 - 8
  • [6] Immobilization of Candida rugosa lipase by sol-gel entrapment and its application in the hydrolysis of soybean oil
    Noureddini, H
    Gao, X
    Joshi, S
    [J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 2003, 80 (11) : 1077 - 1083
  • [7] Synthesis of vitamin E succinate by interfacial activated Candida rugosa lipase encapsulated in sol-gel materials
    Hu, Yi
    Jiang, Xiangjun
    Wu, Suwen
    Jiang, Ling
    Huang, He
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    [J]. COLLOIDS AND SURFACES B-BIOINTERFACES, 2014, 113 : 182 - 189
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    Yilmaz, Elif
    Sezgin, Mehmet
    Yilmaz, Mustafa
    [J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2011, 69 (1-2) : 35 - 41
  • [10] NMR characterization of the role of silane precursors on the catalytic activity of sol-gel encapsulated lipase
    Soares, Cleide M. F.
    dos Santos, Onelia A. A.
    de Castro, Heizir F.
    Itako, Juliana E.
    de Moraes, Flavio F.
    Zanin, Gisella M.
    [J]. JOURNAL OF NON-CRYSTALLINE SOLIDS, 2006, 352 (32-35) : 3469 - 3477
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