A simple assay method for α-amylase using microplates

被引：0
|
作者：
Satoyama, T
Hara, T
Murata, M
Fujio, Y
机构：
[1] Japan Food Res Labs, Kyushu Branch, Hakata Ku, Fukuoka 8120034, Japan
[2] Kyushu Univ, Fac Agr, Inst Genet Resources, Microbial Genet Div,Higashi Ku, Fukuoka 8128581, Japan
[3] Kyushu Univ, Fac Agr, Dept Food Sci & Technol, Higashi Ku, Fukuoka 8128581, Japan
来源：
NIPPON NOGEIKAGAKU KAISHI-JOURNAL OF THE JAPAN SOCIETY FOR BIOSCIENCE BIOTECHNOLOGY AND AGROCHEMISTRY | 1998年 / 72卷 / 08期
关键词：
alpha-amylase; assay; microplate; starch; enzyme;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A novel method for measuring alpha-amylase-activity was developed in this study. By adding an authentic alpha-amylase solution to a mixture of agar and gelatinized rice starch, which was fixed in a well of a microplate, the gel turbidity measured at 655 nm was decreased by the alpha-amylase action on the gelatinized starch. The intensity of the alpha-amylase-activity was in proportion logarithmically to the turbidity decrease detected by a microplate-reader. From reasonable values of standard deviation and coefficient of variation (3.7 similar to 8.9%) of the obtained data, this method makes it possible to measure alpha-amylase-activity conveniently.
引用
收藏
页码：933 / 936
页数：4
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