Force probing of protein crystals: An atomic force microscopy study

The atomic force microscope (AFM) was used for measuring force-distance curves on horse spleen ferritin crystals in liquid environment. In the region of the approach curve which corresponds to tip-surface contact, discrete jumps were recorded, as predicted by molecular dynamics simulations in the case of low tip-sample interaction. The observed jumps can be related to the removal of individual molecules from the surface by the AFM tip. A simple steric model, which takes into account tip and ferritin molecule size; can explain the displacements observed with excellent agreement. The elemental force jump resulting from the approach curves is a direct measure of the force required to remove a single molecule from the crystal face. Sire discuss the conditions under which the cantilever potential energy difference along the elemental force step provides the energy of extraction of a single molecule. The estimate of the intermolecular binding energy turns out to be in good agreement with the value calculated independently from the surface free energy of ferritin crystals.