Development and characterization of EST-SSR markers for Pinus thunbergii

被引：2
|
作者：
Mukasyaf, Aziz Akbar [1 ]
Tamura, Miho [2 ]
Yamaguchi, Rimi [1 ]
Teshima, Kosuke [3 ]
Watanabe, Atsushi [2 ]
机构：
[1] Kyushu Univ, Fac Agr, Grad Sch Bioresource & Bioenvironm Sci, Dept Forest Environm Sci, Fukuoka, Japan
[2] Kyushu Univ, Fac Agr, Dept Forest Environm Sci, Fukuoka, Japan
[3] Kyushu Univ, Fac Sci, Fukuoka, Japan
来源：
JOURNAL OF FOREST RESEARCH | 2021年 / 26卷 / 06期
关键词：
Expressed sequence tag; next-generation sequencing (NGS); population genetics; Pinus thunbergii; EXPRESSED SEQUENCE TAG; MICROSATELLITE MARKERS; GENERATION;
D O I：
10.1080/13416979.2021.1964152
中图分类号：
S7 [林业];
学科分类号：
0829 ; 0907 ;
摘要：
In the present study, a set of ten Expressed Sequence Tag-Simple Sequence Repeats (EST-SSR) markers have been developed to contribute to the genetic studies of Pinus thunbergii. The number of alleles in the developed EST-SSR markers ranged from one to six. Observed heterozygosity was 0.000 to 0.707; the expected heterozygosity ranged from 0.000 to 0.720. Polymorphism information content (PIC) was ranged from 0.000 to 0.669. The mean of the probability of identity between two unrelated individuals (PI) and two siblings (PI-Sib) were 0.540 and 0.719, respectively. The EST-SSR markers developed in the present study, in combining with the previously developed markers, can help in understanding the genetic diversity and genetic structure of P. thunbergii populations.
引用
收藏
页码：464 / 467
页数：4
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