Therapeutic Effect of Ultrasound Combined With Porous Lipid Clioquinol/PLGA Microbubbles on Ferroptosis in HL-1 Cardiac Cell Induced by Isoproterenol Attack

被引：2

作者：

Li, Nana ^{[1
]}

Dong, Lei ^{[1
]}

Shen, Yuanyuan ^{[2
]}

Wang, Yongling ^{[3
]}

Chang, Liansheng ^{[4
]}

Wu, Hongwei ^{[5
]}

Chang, Yuqiao ^{[1
]}

Li, Menghao ^{[1
]}

Li, Dan ^{[1
]}

Li, Zhaoyi ^{[1
]}

He, Mei ^{[1
]}

Li, Cheng ^{[1
]}

Wei, Yao ^{[1
]}

Xie, Haiqin ^{[6
]}

Wang, Feng ^{[1
]}

机构：

[1] Xinxiang Med Univ, Sch Basic Med Sci, Henan Key Lab Med Tissue Regenerat, Xinxiang, Peoples R China

[2] Shenzhen Univ, Hlth Sci Ctr, Sch Biomed Engn, Natl Reg Key Technol Engn Lab Med Ultrasound, Shenzhen, Peoples R China

[3] Xinxiang Med Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Xinxiang, Peoples R China

[4] Xinxiang Med Univ, Sch Basic Med Sci, Dept Human Anat Histol & Embryol, Xinxiang, Peoples R China

[5] Xinxiang Med Univ, Dept Chem, Xinxiang, Peoples R China

[6] Peking Univ, Dept Ultrasound, Shenzhen Hosp, Shenzhen, Peoples R China

来源：

FRONTIERS IN PHARMACOLOGY | 2022年 / 13卷

基金：

国家重点研发计划;

关键词：

ferroptosis; clioquinol; microbubbles; low-frequency ultrasound; cardiovascular diseases; PLGA;

D O I：

10.3389/fphar.2022.918292

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In recent years, studies have shown a close relationship between cardiomyocyte death and ferroptosis. Clioquinol (CQ) can inhibit ferroptosis. Porous lipid-poly (lactic-co-glycolic acid) (PLGA) microbubbles (MBs) were prepared by double emulsification (W-1/O/W-2) using 1,2-dioctadecanoyl-sn-glycero-3-phophocholine and PLGA as raw materials. Porous lipid-PLGA MBs were used as carriers to prepare CQ/PLGA MBs containing CQ. CQ/PLGA had the advantages of high drug loading, good biocompatibility, and sustained release. Our results showed that CQ/PLGA improved the effect of CQ and reduced its cytotoxicity. Under low-frequency ultrasound with certain parameters, CQ/PLGA showed steady-state cavitation, which increased the membrane permeability of mouse cardiomyocyte HL-1 to a certain extent and further prevented the process of ferroptosis in mouse cardiomyocyte HL-1.

引用

页数：11