The use of RAPD technique for the identification and classification of Pisum sativum L genotypes

被引：27
|
作者：
Samec, P
Nasinec, V
机构：
[1] Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, 370 05 Ceske Budejovice
来源：
EUPHYTICA | 1996年 / 89卷 / 02期
关键词：
cultivar identification; genetic relatedness; Pisum sativum; RAPD; pea;
D O I：
10.1007/BF00034610
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
The genomic DNAs of 42 Pisum sativum genotypes, representing four wild and cultivated subspecies were used as templates in RAPD reactions. Amplification with eight decamer primers generated 149 polymorphic products. Genetic similarities of RAPD profiles were estimated via a coefficient of Jaccard and then the data were processed by cluster analysis (UPGMA). Each genotype was clearly identified and separated from the others. Our results show that RAPD technology is a rapid, precise and sensitive technique for identification of pea genotypes. However, the phylogenetic relationships within the Pisum sativum, which we tested by bootstrap analysis (Wagner parsimony), must be interpreted with caution.
引用
收藏
页码：229 / 234
页数：6
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