The single crystal of ZnWO4:Ho3+ was grown by Czochralski technique. The XRD, absorption spectra, fluorescence spectrum are presented, and the Judd - Ofelt (J - O) intensity parameters Omega(2), Omega(4), Omega(6) are obtained to be 4.61 x 10(-20) cm(2), 0.16 x 10(-20) cm(2), 0.48 x 10(-20) cm(2), respectively. Calculated radiative transition rate, branching ratios, and radiative lifetime for different transition levels of ZnWO4:Ho3+ crystals are presented. The most intense line correlative with the transition S-5(2)-> I-5(8) at 543 nm is potentially used for green solid state laser, and the fluorescence lifetime of this energy level is 14.1 mu s. (c) 2007 Elsevier B.V. All rights reserved.