Presence of the NHE(3) isoform of the Na+/H+ exchanger in human gallbladder

被引:8
|
作者
Colombani, V
Silviani, V
Marteau, C
Lerique, B
Cartouzou, G
Gerolami, A
机构
[1] FAC MED MARSEILLE,INSERM,F-13385 MARSEILLE 05,FRANCE
[2] HOP CONCEPTION,MARSEILLE,FRANCE
关键词
human gallbladder; sodium absorption; sodium/hydrogen exchanger;
D O I
10.1042/cs0910209
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
1. In man and in various animal species, absorption of NaCl from bile by the gallbladder mucosa is associated with luminal proton secretion, A similar absorption of NaCl in small intestine, colon and renal tubule is related, at least in part, to the presence of the NHE(3) isoform of the Na+/H+ exchanger. This work was designed to find out whether NHE(3) is also present in human gallbladder. 2. At surgery, 100-200 mg of the gallbladder wall was obtained from patients treated by cholecystectomy for gallstones. After isolation of the mucosa, total RNA was extracted and submitted to reverse transcription-polymerase chain reaction with two primers: 5'-AAGCCICTGGTGCAGTGGCTGAAGG-3' and 5'-GGAGTCCTTIAAGTCGGCIAAGCTGGGC-3', designed to amplify a sequence of 645 bp of rabbit NHE(3) mRNA (642 bp in man), RNA from human liver and from rabbit heart, neither of which, contain NHE(3), and human ileal RNA, which does contain NHE(3), were used as controls. 3. RNA extracted from the mucosal moiety of the gallbladder wall gave an amplification product of about 645 nucleotides, Controls gave the expected negative or positive results. Sequencing of the amplified RNA showed it was almost identical to previously determined sequences of NHE(3) in other human tissues. 4. It is concluded that the mucosa of human gallbladder contains the mRNA of NHE(3) isoform. This isoform could therefore play a role in sodium absorption from bile.
引用
收藏
页码:209 / 212
页数:4
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