Development and applications of a bovine coronavirus antigen detection enzyme-linked immunosorbent assay

被引：16
|
作者：
Schoenthaler, SL [1 ]
Kapil, S [1 ]
机构：
[1] Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA
来源：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 1999年 / 6卷 / 01期
关键词：
D O I：
10.1128/CDLI.6.1.130-132.1999
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
We developed a monoclonal antibody-based, antigen capture sandwich enzyme-linked immunosorbent assay (ELISA) for bovine coronavirus. We compared the ELISA with electron microscopy and the hemagglutination test and found a close correlation between them. The sensitivity of the ELISA was 10(4) bovine coronavirus particles per ml of 10% fecal suspension. Compared with electron microscopy, bovine coronavirus ELISA had 96% specificity.
引用
收藏
页码：130 / 132
页数：3
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