Macrocyclic Antibiotics as Chiral Selectors in High-Performance Liquid Chromatography and Capillary Electrophoresis

Data on the use of macrocyclic antibiotics (vancomycin, teicoplanin, teicoplanin aglycone, and eremomycin) for the enantioseparation of amino acids, various amino acid derivatives, -phenylcarboxylic acids, -blockers, and some pharmaceutical preparations in reversed-phase and polar-organic HPLC modes are summarized. It is shown that mixed chiral selectors (eremomycin-vancomycin, eremomycin-bovine serum albumin) combine the properties of two selectors. Eremomycin and macrolides (azithromycin, erythromycin, and clarithromycin) are successfully used as chiral selectors in capillary electrophoresis. Aqueous and aqueous-organic supporting electrolytes (SEs) with the addition of eremomycin or nonaqueous supporting electrolytes with the addition of a macrolide are used for enantioseparation. The use of nonaqueous supporting electrolytes decreases the adsorption of the selectors on the quartz capillary surface and enables the separation of enantiomers at a low concentration of a chiral selector. Additions of boric acid into the supporting electrolyte improve the selectivity of separation.