Expression, purification and characterization of recombinant α-glucosidase in Pichia pastoris

被引：10
|
作者：
Wu, K. Y. [2 ]
Huang, S. H. [1 ]
Ding, S. [1 ]
Zhang, Y. K. [1 ]
Chen, G. G. [1 ]
Liang, Z. Q. [1 ]
机构：
[1] Guangxi Univ, Coll Life Sci & Technol, Nanning 530005, Peoples R China
[2] Guangxi Univ, Inst Light Ind & Food Engn, Nanning 530005, Peoples R China
来源：
FOLIA MICROBIOLOGICA | 2010年 / 55卷 / 06期
关键词：
ASPERGILLUS-NIGER; PANOSE;
D O I：
10.1007/s12223-010-0093-7
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An expression plasmid containing the agdA gene encoding Aspergillus oryzae ZL-1 alpha-glucosidase was constructed and expressed in Pichia pastoris X-33. The molar mass of the purified protein was estimated by SDS-PAGE. HPLC analysis showed that the purified enzyme has a transglucosylating activity with maltose as substrate. The main component of the enzyme products was panose, while amounts of isomaltose and isomaltotriose were very low or absent. pH 5.2 and temperature of 37 A degrees C were optimum for enzyme activity.
引用
收藏
页码：582 / 587
页数：6
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