Enantioselective determination of isradipine in human serum using chiral stationary phase liquid chromatography and gas chromatography with nitrogen selective detection

被引:0
|
作者
Rask, HS [1 ]
Angelo, HR [1 ]
Christensen, HR [1 ]
机构
[1] Bispebjerg Hosp, Dept Clin Biochem, Unit Clin Pharmacol, DK-2400 Copenhagen NV, Denmark
关键词
calcium antagonist; stereoselective analytical method; isradipine enantiomers; Chiral OJ; HPLC; GC;
D O I
10.1002/(SICI)1520-636X(1998)10:9<808::AID-CHIR6>3.0.CO;2-H
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
rac-Isradipine is a dihydropyridine type calcium antagonist. Its calcium entry blocking effect is due primarily to the (+)-(S)-enantiomer. This study describes a sensitive enantioselective method for the determination of isradipine in human serum. Following alkaline extraction into hexane, the enantiomers of isradipine are separated quantitatively by high-performance liquid chromatography on a Chiralcel OJ column at 39 degrees C. The collected fractions were evaporated and assayed using capillary gas chromatography on a HP 50+ column with nitrogen selective detection. Using 2.0 ml of serum, 0.7 nmol/l (0.26 ng/ml) of each enantiomer could be determined with acceptable precision. The method has successfully been used to measure (+)-(S)- and (-)-(R)-isradipine concentrations in samples from volunteers after intravenous and oral administration of isradipine. (C) 1998 Wiley-Liss, Inc.
引用
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页码:808 / 812
页数:5
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