Development of a simple high-throughput assay for directed evolution of enantioselective sulfoxide reductases

被引：9

作者：

Tarallo, Vincenzo ^{[1
]}

Sudarshan, Kasireddy ^{[1
]}

Nosek, Vladimir ^{[1
]}

Misek, Jiri ^{[1
]}

机构：

[1] Charles Univ Prague, Fac Sci, Dept Organ Chem, Hlavova 2030-8, Prague 12843 2, Czech Republic

来源：

CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 40期

关键词：

METHIONINE; DNA; BIOCATALYSIS; ALBENDAZOLE; ENZYME; GENES;

D O I：

10.1039/d0cc01660h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report on the development of high-throughput fluorogenic assay that can streamline directed evolution of enantioselective sulfoxide reductases. As a model, methionine sulfoxide reductase A (MsrA) has been evolved to expand its limited substrate scope. The resulting mutant MsrA can resolve a range of new challenging racemic sulfoxides with high efficiency including the pharmaceutically relevant albendazole sulfoxide. The simplicity and the level of throughput make this method also suitable for the screening of metagenomic libraries in future for the discovery of new enzymes with similar reactivities.

引用

页码：5386 / 5388

页数：3

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