Identification of cardiac marker protein during ischemia and reperfusion using two dimensional gel electrophoresis and matrix-assisted laser desorption ionization mass Spectrometry

The purpose of the present study was to evaluate difference of the cardiac marker protein expression in 3 types of heart tissue (control, ischemic preconditioning (IPC), and ischemia-reperfusion) using proteomic analysis. Appearance in expression of one protein was observed in ischemia-reperfusion heart gel, but the spot was not detected in control and IPC heart gels. We were able to identify the spot is myoglobin, which was confirmed by Western immunoblot. Our result was consistent with previous studies of cardiac marker in ischemic heart disease and showed that myoglobin is suitable cardiac marker as measure of myocardial injury. In addition, through the multiple techniques, including 2DE, MALDI-MS, and Western immunoblot, proteomic analysis is appropriate means of the identification of cardiac marker in study of IPC and ischemia-reperfusion.