Demonstrating polymorphic miRNA-mediated gene regulation in vivo: Application to the g+6223G→A mutation of Texel sheep

被引：11

作者：

Takeda, Haruko ^{[1
]}

Charlier, Carole ^{[1
]}

Farnir, Frederic ^{[2
]}

Georges, Michel ^{[1
]}

机构：

[1] Univ Liege, Fac Vet Med, GIGA Res Ctr, Unit Anim Genom, B-4000 Liege, Belgium

[2] Univ Liege, Fac Vet Med, GIGA Res Ctr, Unit Biostat, B-4000 Liege, Belgium

来源：

RNA | 2010年 / 16卷 / 09期

关键词：

AGO immunoprecipitation; allelic imbalance; microRNA target; polymorphic miRNA-mediated gene regulation; SPASTIC PARAPLEGIA TYPE-31; PROGRESSIVE HEARING-LOSS; MICRORNA TARGET SITE; MESSENGER-RNA; SYSTEMATIC IDENTIFICATION; UNTRANSLATED REGION; BINDING SITES; BREAST-CANCER; RISK; ASSOCIATION;

D O I：

10.1261/rna.2131110

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We herein describe the development of a biochemical method to evaluate the effect of single nucleotide polymorphisms (SNPs) in target genes on their regulation by microRNAs in vivo. The method is based on the detection of allelic imbalance in RNAs coimmunoprecipitated with AGO proteins from tissues of heterozygous individuals. We characterize the performances of our approach using a model system in a cell culture, and then apply it successfully to prove that the 3'UTR g+6223G -> A mutation operates by promoting RISC-dependent down-regulation of myostatin (MSTN) in skeletal muscle of Texel sheep.

引用

页码：1854 / 1863

页数：10

共 3 条

[1] Patrocles: a database of polymorphic miRNA-mediated gene regulation in vertebrates
Hiard, Samuel
Charlier, Carole
Coppieters, Wouter
Georges, Michel
Baurain, Denis
NUCLEIC ACIDS RESEARCH, 2010, 38 : D640 - D651
[2] Polymorphic miRNA-mediated gene regulation: contribution to phenotypic variation and disease
Georges, Michel
Coppieters, Wouter
Charlier, Carole
CURRENT OPINION IN GENETICS & DEVELOPMENT, 2007, 17 (03) : 166 - 176
[3] Preferential potentiation of AMPA-mediated currents in brainstem hypoglossal motoneurons by subchronic exposure of mice expressing the human superoxide dismutase 1 G93A gene mutation to neurotoxicant methylmercury in vivo
Yuan, Yukun
Bailey, Jordan M.
Rivera-Lopez, Gretchen M.
Atchison, William D.
NEUROTOXICOLOGY, 2024, 100 : 72 - 84

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