Real time TaqMan RT-PCR assay for the detection of Cucumber green mottle mosaic

被引：49

作者：

Chen Hongyun ^{[1
,2
]}

Zhao Wenjun ^{[2
]}

Gu Qinsheng ^{[3
]}

Chen Qing ^{[1
]}

Lin Shiming ^{[1
]}

Zhu Shuifang ^{[2
]}

机构：

[1] Xiamen Entry Exit Inspect & Quarantine Bur, Xiamen 361012, Peoples R China

[2] Chinese Acad Inspect & Quarantine, Beijing 100029, Peoples R China

[3] Chinese Acad Agr Sci, Zhengzhou Fruit Res Inst, Zhengzhou 450009, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2008年 / 149卷 / 02期

关键词：

cucumber green mottle mosaic virus; tobamovirus; real time RT-PCR; quarantine virus;

D O I：

10.1016/j.jviromet.2008.02.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A real time reverse-transcription polymerase chain reaction (RT-PCR) was developed for efficient detection of Cucumber green mottle mosaic virus (CGMMV). The method was designed to use a duo-primer system with a TaqMan probe targeting the conserved sequence in 3' noncoding region (NCR) of CGMMV to detect isolates of this virus collected in China. The sensitivity of the real time RT-PCR assay was 0.13 pg of total RNA or 50 molecules of RNA transcripts. This level of sensitivity indicated that the one step real time RT-PCR developed in the present study could be used for routine testing assays. The real time RT-PCR method could assist in the implementation of quarantine measures for prevention and control of the disease caused by CGMMV. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：326 / 329

页数：4

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