Enhanced peroxidase-like activity of Fe 3 O 4-sodium lignosulfonate loaded copper peroxide composites for colorimetric detection of H 2 O 2 and glutathione

In this paper, the composites of Fe3O4 modified by sodium lignosulfonate and copper peroxide (Fe3O4@CP) were produced by a simple two-step method, and their morphology and composition were featured in Field emission scanning electron microscopy, X-ray powder diffraction, X-ray diffraction and Fourier transform infrared. Then, Fe3O4@CP catalyzed the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine to blue oxide in the presence of H2O2, indicating that it had good catalytic performance. Further, the experimental conditions were optimized, including time, pH, temperature and material concentration. The kinetic analysis results showed that Fe3O4@CP exhibited excellent catalytic performance and its catalytic kinetic plot conformed to the Michaelis-Menten equation and the catalytic mechanism was consistent with the ping-pong mechanism. Finally, a H2O2 colorimetric assay with a linear range of 0.2–300 μM and a detection limit of 0.11 μM was constructed. In addition, due to the decolorization reaction of ox-TMB with glutathione and the scavenging effect of GSH on hydroxyl radicals (·OH), a glutathione colorimetric assay was further constructed with a linear range of 0.2–40 μM and a detection limit of 0.05 μM. It also verified that the assay had excellent selectivity and stability and could be utilized to detect actual samples. © 2020 Elsevier B.V.