Real-time polymerase chain reaction (PCR) for the study of ancient DNA.

被引：5
|
作者：
Kefi, R
Mafart, B
Spadoni, JL
Stevanovitch, A
Beraud-Colomb, T
机构：
[1] Hop St Marguerite, INSERM, U 387, F-13277 Marseille 9, France
[2] Fac Med Secteur Nord Marseille, Lab Anthropol, F-13015 Marseille, France
来源：
COMPTES RENDUS PALEVOL | 2003年 / 2卷 / 02期
关键词：
ancient DNA; mitochondrial DNA; HVSI region; real-time PCR; cleaning bone method;
D O I：
10.1016/S1631-0683(03)00029-0
中图分类号：
Q91 [古生物学];
学科分类号：
0709 ; 070903 ;
摘要：
Real-time Polymerase Chain Reaction (PCR) for the study of ancient DNA. The properties of ancient DNA (aDNA) make difficult the retrieval of DNA sequence. The advantage of Real-Time PCR was exploited, for the first time, in the study of aDNA. We determined the optimal condition to amplify, in one round of PCR, aDNA, which should be directly sequenced. Beside the verification of aDNA authenticity, we compared two cleaning bone methods: scalpel and ethanol. The ethanol specimens showed the best DNA yield. The aDNA was extracted and amplified (mitochondrial hypervariable region 1) from five skeletons exhumed from the archaeological site of Notre-Dame-du-Bourg (France), dated from 3rd to 17th century. (C) 2003 Editions scientifiques et medicales Elsevier SAS. All rights reserved.
引用
收藏
页码：125 / 132
页数：8
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