Enzyme-mediated polymerization inside engineered protein cages

被引：31
|
作者：
Frey, Raphael [1 ]
Hayashi, Takahiro [1 ]
Hilvert, Donald [1 ]
机构：
[1] ETH, Organ Chem Lab, CH-8093 Zurich, Switzerland
来源：
CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 68期
基金：
欧洲研究理事会; 日本学术振兴会;
关键词：
TRANSFER RADICAL POLYMERIZATION; RING-OPENING POLYMERIZATION; BACTERIAL MICROCOMPARTMENTS; ORGANELLES; CONTAINER; CARGO; NANOPARTICLES; ENCAPSULATION; PEROXIDASE; EVOLUTION;
D O I：
10.1039/c6cc05301g
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Engineered variants of the capsid-forming enzyme lumazine synthase, AaLS, were used as nanoreactors for an enzyme-mediated polymerization. Oxidation of 3,3-diaminobenzidine (DAB) by the engineered ascorbate peroxidase APEX2 encapsulated in AaLS capsids resulted in templated formation of polyDAB-capsid nanoparticles of homogeneous size and shape.
引用
收藏
页码：10423 / 10426
页数：4
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