Enhanced cadaverine production from L-lysine using recombinant Escherichia coli co-overexpressing CadA and CadB

被引：73
|
作者：
Ma, Weichao [1 ,2 ]
Cao, Weijia [1 ]
Zhang, Hong [1 ]
Chen, Kequan [1 ]
Li, Yan [1 ]
Ouyang, Pingkai [1 ]
机构：
[1] Nanjing Tech Univ, State Key Lab Mat Oriented Chem Engn, Coll Biotechnol & Pharmaceut Engn, Nanjing 211816, Jiangsu, Peoples R China
[2] Tianshui Normal Univ, Sch Bioengn & Biotechnol, Tianshui 741001, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2015年 / 37卷 / 04期
关键词：
Cadaverine; Co-expression; Lysine/cadaverine antiporter; PelB; Whole-cell bioconversion; CORYNEBACTERIUM-GLUTAMICUM; E; COLI; PERMEABILITY; ANTIPORTER; SECRETION; PROTEINS; STRESS; AMINES; OPERON; LEADS;
D O I：
10.1007/s10529-014-1753-5
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The effect of fusing the PelB signal sequence to lysine/cadaverine antiporter (CadB) on the bioconversion of l-lysine to cadaverine was investigated. To construct a whole-cell biocatalyst for cadaverine production, four expression plasmids were constructed for the co-expression of lysine decarboxylase (CadA) and lysine/cadaverine antiporter (CadB) in Escherichia coli. Expressing CadB with the PelB signal sequence increased cadaverine production by 12 %, and the optimal expression plasmid, pETDuet-pelB-CadB-CadA, contained two T7 promoter-controlled genes, CadA and the PelB-CadB fusion protein. Based on pETDuet-pelB-CadB-CadA, a whole-cell system for the bioconversion of l-lysine to cadaverine was constructed, and three strategies for l-lysine feeding were evaluated to eliminate the substrate inhibition problem. A cadaverine titer of 221 g l(-1) with a molar yield of 92 % from lysine was obtained.
引用
收藏
页码：799 / 806
页数：8
相关论文
共 50 条
  • [1] Enhanced cadaverine production from l-lysine using recombinant Escherichia coli co-overexpressing CadA and CadB
    Weichao Ma
    Weijia Cao
    Hong Zhang
    Kequan Chen
    Yan Li
    Pingkai Ouyang
    Biotechnology Letters, 2015, 37 : 799 - 806
  • [2] Biotransformation of lysine into cadaverine using barium alginate-immobilized Escherichia coli overexpressing CadA
    Shashi Kant Bhatia
    Yong Hyun Kim
    Hyun Joong Kim
    Hyung-Min Seo
    Jung-Ho Kim
    Hun-Seok Song
    Ganesan Sathiyanarayanan
    Sung-Hee Park
    Kyungmoon Park
    Yung-Hun Yang
    Bioprocess and Biosystems Engineering, 2015, 38 : 2315 - 2322
  • [3] Biotransformation of lysine into cadaverine using barium alginate-immobilized Escherichia coli overexpressing CadA
    Department of Biological Engineering, College of Engineering, Konkuk University, Seoul
    143-701, Korea, Republic of
    不详
    152-051, Korea, Republic of
    不详
    339-701, Korea, Republic of
    不详
    143-701, Korea, Republic of
    Bioprocess Biosyst. Eng., 12 (2315-2322):
  • [4] Biotransformation of lysine into cadaverine using barium alginate-immobilized Escherichia coli overexpressing CadA
    Bhatia, Shashi Kant
    Kim, Yong Hyun
    Kim, Hyun Joong
    Seo, Hyung-Min
    Kim, Jung-Ho
    Song, Hun-Seok
    Sathiyanarayanan, Ganesan
    Park, Sung-Hee
    Park, Kyungmoon
    Yang, Yung-Hun
    BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2015, 38 (12) : 2315 - 2322
  • [5] Effective synthesis of cadaverine from L-lysine using recombinant decarboxylase under CO2 purging condition
    Jeong, Seongwook
    Choi, Eun-Gyu
    Byun, Sungmin
    Cho, Dae Haeng
    Kim, Yong Hwan
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2015, 249
  • [6] Characterization of a Whole-Cell Biotransformation Using a Constitutive Lysine Decarboxylase from Escherichia coli for the High-Level Production of Cadaverine from Industrial Grade l-Lysine
    Jihyun Shin
    Jeong Chan Joo
    Eunji Lee
    Sung Min Hyun
    Hyun Joong Kim
    Si Jae Park
    Yung-Hun Yang
    Kyungmoon Park
    Applied Biochemistry and Biotechnology, 2018, 185 : 909 - 924
  • [7] High-level L-lysine bioconversion into cadaverine with enhanced productivity using engineered Escherichia coli whole-cell biocatalyst
    Leong, Yoong Kit
    Chen, Chien-Heng
    Huang, Shih-Fang
    Lin, Hung-Yi
    Li, Sheng-Feng
    Ng, I-Son
    Chang, Jo-Shu
    BIOCHEMICAL ENGINEERING JOURNAL, 2020, 157
  • [8] Characterization of a Whole-Cell Biotransformation Using a Constitutive Lysine Decarboxylase from Escherichia coli for the High-Level Production of Cadaverine from Industrial Grade l-Lysine
    Shin, Jihyun
    Joo, Jeong Chan
    Lee, Eunji
    Hyun, Sung Min
    Kim, Hyun Joong
    Park, Si Jae
    Yang, Yung-Hun
    Park, Kyungmoon
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2018, 185 (04) : 909 - 924
  • [9] Optimizing Escherichia coli strains and fermentation processes for enhanced L-lysine production: a review
    Wu, Zijuan
    Chen, Tianpeng
    Sun, Wenjun
    Chen, Yong
    Ying, Hanjie
    FRONTIERS IN MICROBIOLOGY, 2024, 15
  • [10] High-Level Conversion of L-lysine into Cadaverine by Escherichia coli Whole Cell Biocatalyst Expressing Hafnia alvei L-lysine Decarboxylase
    Kim, Hee Taek
    Baritugo, Kei-Anne
    Oh, Young Hoon
    Kang, Kyoung-Hee
    Jung, Ye Jean
    Jang, Seyoung
    Song, Bong Keun
    Kim, Il-Kwon
    Lee, Myung Ock
    Hwang, Yong Taek
    Park, Kyungmoon
    Park, Si Jae
    Joo, Jeong Chan
    POLYMERS, 2019, 11 (07)
12345