Rubia Gallega x Nelore crossbred cattle improve beef tenderness through changes in protein abundance and gene expression

被引：1

作者：

Ferrinho, Adrielle M. ^{[1
]}

de Moura, Gabriella Vespe ^{[1
]}

Martins, Taiane da Silva ^{[2
]}

Munoz, Julian ^{[1
]}

Mueller, Lenise F. ^{[1
]}

Garbossa, Pollyana Leite Matioli ^{[2
]}

de Amorim, Tamyres Rodrigues ^{[1
]}

Jessica, Luana Gemelli ^{[1
]}

Fuzikawa, Ingrid Harumi de Souza ^{[1
]}

Prado, Cibele ^{[1
]}

da Silveira, Juliano Coelho ^{[1
]}

Poleti, Mirele D. ^{[1
]}

Baldi, Fernando ^{[3
]}

Pereira, Angelica S. C. ^{[1
,2
]}

机构：

[1] Univ Sao Paulo, USP, Fac Anim Sci & Food Engn, BR-13635900 Pirassununga, SP, Brazil

[2] Univ Sao Paulo, Fac Vet Med & Anim Sci, USP, BR-13635900 Pirassununga, SP, Brazil

[3] Sao Paulo State Univ, Fac Agr & Vet Sci, UNESP, BR-14884000 Jaboticabal, SP, Brazil

来源：

LIVESTOCK SCIENCE | 2021年 / 251卷

基金：

瑞典研究理事会; 巴西圣保罗研究基金会;

关键词：

Bos indicus; Bos taurus; Myostatin; Proteolysis; HSP and troponin-T; HEAT-SHOCK PROTEINS; MEAT QUALITY TRAITS; SKELETAL-MUSCLE; CALPASTATIN ACTIVITY; POSTMORTEM PROTEOLYSIS; MYOFIBRILLAR PROTEIN; LONGISSIMUS-THORACIS; CALPAIN SYSTEM; MYOSTATIN GENE; BOS-INDICUS;

D O I：

10.1016/j.livsci.2021.104634

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The objective was to evaluate meat quality of Rubia Gallega x Nelore (RGN) crossbred compared to Nelore (N) cattle, using mRNA analysis of calpain 1 and 2, calpastatin, myostatin, and TGF beta; and protein abundance profile post mortem based on aged beef of the different genetic groups. Sixteen N and 16 RGN were finished in feedlot by 120 days and harvested. RGN beef presented greater tenderness. Transcripts analysis showed increased levels of TGF beta and MSTN in RGN, indicating differences in muscle fiber formation. However, no difference was observed in mRNA for CAPN1, CAPN2 and CAST. Protein analysis demonstrated increased troponin-T degradation in RGN. Interaction for HSP 27 degradation was observed, indicating the degradation of this protein is faster and greater in RGN than N. No difference in myostatin protein abundance was found. In general, RGN cattle improves beef quality and these differences may be explained by TGF beta and MSTN gene expression, troponin-T degradation and HSP abundance.

引用

页数：13