Sequence Specific DNA Cross-Linking Triggered by Visible Light

被引：71
|
作者：
Op de Beeck, Marieke [1 ]
Madder, Annemieke [1 ]
机构：
[1] Univ Ghent, Lab Organ & Biomimet Chem, B-9000 Ghent, Belgium
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2012年 / 134卷 / 26期
关键词：
SINGLET OXYGEN; MOLECULAR-OXYGEN; OXIDATION; RNA;
D O I：
10.1021/ja301901p
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A new biocompatible strategy for photoinduced DNA interstrand cross-linking is presented. Methylene blue induced O-1(2) formation triggers furan oxidation; the resulting aldehyde then rapidly reacts with complementary A or C with formation of stable adducts. Easily accessible furan modified nucleosides, a commercially available photosensitizer, and visible light irradiation constitute the necessary tools to achieve selective duplex interstrand cross-linking.
引用
收藏
页码：10737 / 10740
页数：4
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