A Microchip for High-Throughput Axon Growth Drug Screening

被引:12
|
作者
Kim, Hyun Soo [1 ]
Jeong, Sehoon [2 ]
Koo, Chiwan [3 ]
Han, Arum [1 ,2 ]
Park, Jaewon [1 ,4 ]
机构
[1] Texas A&M Univ, Dept Elect & Comp Engn, College Stn, TX 77843 USA
[2] Texas A&M Univ, Dept Biomed Engn, College Stn, TX 77843 USA
[3] Hanbat Natl Univ, Dept Elect & Control Engn, Daejeon 305719, South Korea
[4] Southern Univ Sci & Technol, Neural & Cognit Sci Res Ctr, Dept Elect & Elect Engn, Shenzhen 518055, Peoples R China
来源
MICROMACHINES | 2016年 / 7卷 / 07期
基金
美国国家卫生研究院;
关键词
microfluidic neuron culture platform; compartmentalized culture; localized biomolecular treatment; high-throughput screening; MICROFLUIDIC CULTURE PLATFORM; RETINAL GANGLION-CELLS; QUANTITATIVE-ANALYSIS; GLIAL INHIBITION; REGENERATION; CNS; NEURONS; INJURY; FABRICATION; COCULTURE;
D O I
10.3390/mi7070114
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
It has been recently known that not only the presence of inhibitory molecules associated with myelin but also the reduced growth capability of the axons limit mature central nervous system (CNS) axonal regeneration after injury. Conventional axon growth studies are typically conducted using multi-well cell culture plates that are very difficult to use for investigating localized effects of drugs and limited to low throughput. Unfortunately, there is currently no other in vitro tool that allows investigating localized axonal responses to biomolecules in high-throughput for screening potential drugs that might promote axonal growth. We have developed a compartmentalized neuron culture platform enabling localized biomolecular treatments in parallel to axons that are physically and fluidically isolated from their neuronal somata. The 24 axon compartments in the developed platform are designed to perform four sets of six different localized biomolecular treatments simultaneously on a single device. In addition, the novel microfluidic configuration allows culture medium of 24 axon compartments to be replenished altogether by a single aspiration process, making high-throughput drug screening a reality.
引用
收藏
页数:13
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