O6-methylguanine DNA methyltransferase activity in diabetic patients

被引：7
|
作者：
Akçay, T
Dinçer, Y
Çelebi, N
Ilkova, H
机构：
[1] Istanbul Univ, Cerrahpasa Med Sch, Dept Biochem, Istanbul, Turkey
[2] Cekmece Nucl Res & Training Ctr, Istanbul, Turkey
[3] Cerrahpasa Med Sch, Dept Internal Med, Div Endocrinol Metab & Diabet, Istanbul, Turkey
来源：
DIABETES RESEARCH AND CLINICAL PRACTICE | 2003年 / 61卷 / 01期
关键词：
O-6-methylguanine; O-6-methylguanine DNA methyltransferase; leukocyte; diabetes mellitus; HbA(1c);
D O I：
10.1016/S0168-8227(03)00063-9
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
In the present study, we evaluated O-6-methylguanine-DNA methyltransferase (MGMT) activity in diabetic patients. The study was performed on 27 patients with Type 1 diabetes, and 42 with Type 2 diabetes. Patients with complications were excluded from the study. 36 non-diabetic volunteers, non-smokers who do not consume alcoholic beverage, were chosen from the medical staff as control subjects. MGMT activity was measured by the transfer of radiolabeled methyl groups from a prepared methylguanine-DNA substrate to the enzyme fraction of leukocyte extract. Leukocyte MGMT activity was significantly reduced in both Type 1 and Type 2 diabetes patients as compared with control subjects (P < 0.001). The present study demonstrates decreased MGMT activity in leukocytes from patients with Type 1 and Type 2 diabetes. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码：1 / 6
页数：6
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