Molecular Detection of Anaplasma phagocytophilum in Ixodid Ticks in Hebei Province, China

被引:14
|
作者
Zou Yaxue [2 ]
Jin Hongtao [1 ]
Wang Qiuyue [2 ]
Fu Zhixin [2 ]
Gao Hongwei [1 ]
Liu Pengpeng [2 ]
Liu Quan [1 ]
Chen Lifeng [2 ]
机构
[1] Acad Mil Med Sci, Inst Mil Vet, Key Lab Jilin Prov Zoonosis Prevent & Control, Changchun 130122, Jilin Province, Peoples R China
[2] Hebei Normal Univ Sci & Technol, Key Lab Prevent Vet Med Hebei Prov, Qinhuangdao 066000, Peoples R China
关键词
Anaplasma phagocytophilum; Dermacentor nuttalli; Haemaphysalis longicornis; nested PCR; HUMAN GRANULOCYTIC ANAPLASMOSIS; INFECTION; EHRLICHIA; JAPAN; TRANSMISSION; PERSULCATUS; PREVALENCE; MARGINALE; ACARI; AREA;
D O I
10.1089/vbz.2010.0253
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
A total of 3696 Ixodid ticks, collected from Hebei Province, China, were examined by a nested polymerase chain reaction for the presence of Anaplasma phagocytophilum. Forty-three (15.4%) of 280 pools tested, including 39 (14.6%) of 267 Haemaphysalis longicornis and four (30.8%) of 13 Dermacentor nuttalli, were positive, but no significant difference was found between D. nuttalli and H. longicornis (p > 0.05). Sequence and phylogenetic analyses of 16S rRNA gene indicated that A. phagocytophilum in China is genetically diverse. To our knowledge, this is the first evidence of A. phagocytophilum in ticks from Hebei Province, China, and the first documentation of Anaplasma infection in D. nuttalli.
引用
收藏
页码:1323 / 1327
页数:5
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