Determination and analysis of toluene diisocyanate metabolites in mice using gas chromatography-mass spectrometry

In the present research we used gas chromatography-mass spectrometry (GC-MS) to determine metabolites of toluene diisocyanate (TDI) in mice and deduce the pathway for toluene diisocyanate metabolism in the organism. Conditions for TDI chromatography: Supelco PTETM-5 chromatographic column (30 mmx 0. 25 mmx 0. 25 pm); initial column temperature: 90 V, which was maintained for 30 min, then the temperature was increased at a rate of 40 V center dot min(-1) to 280 degrees C, and maintained for 5. 25 min; temperature for the vaporizing chamber: 250 V; carrier gas: helium flowing at 1. 0 mu L center dot min(-1). Conditions for chromatography of TDI metabolites in the organism: 94% methyl, 4% ethenyl-bonded-phase fused-silica capillary column (30+ 2 mX0. 25 + 0. 02 nim); initial column temperature: 30 degrees C, which was maintained for 5 min, after and then was increased at a rate of 80 degrees C center dot min(-1) to 280 degrees C, and maintained for 5 min; temperature for the vaporizing chamber: 250 degrees C; carrier gas: helium flowing at 1. 0 mu L center dot min(-1). Conditions for mass spectrometry: El for ionization; 70 eV for ionization energy; 280 degrees C for connecting tube temperature; 35-350 mu for range of scanning; and 1. 0 mu L for sample size. The results showed that 2,4-toluene diisocyanate was metabolized into 2,4-diaminotoluene. Under the conditions selected for GC-MS, TDI metabolites in the organism can be isolated and identified.