Various fractions of Hypericum x moserianum and Hypericum ericoides possess antiglycation, anti-lipid peroxidation, antioxidative activities and non-toxic effects in vitro

In the present study, two species Hypericum x moserianum and Hypericum ericoides which belong to genus Hypericum were evaluated for their potential antiglycation, antioxidant, anti lipid peroxidation and cytotoxic activities. These species are widely used in folk medicine and to the best of our knowledge. there were no previous reports regarding antioxidant, anti-glycation and cytotoxicity studies of these species. Among the crude methanol extracts and fractions of both the Species, the ethyl acetate fraction of H. x moserianum exhibited promising antioxidant activity against 1,1'-diphenyl-2-picrylhydrazyl (DPPH) with IC50 129.084 +/- 1.215 mu g/ml, followed by methanol extract (IC50=232.083 +/- 1.215 mu g/ml) and aqueous fraction (IC50=266.962 +/- 2.213 mu g/ml). The ethyl acetate ftaction of H. ericoides exhibited IC50 value of 295.088 +/- 2.320 mu g/ml. In antiglycation assay, the ethyl acetate fraction of H. x moserianum showed 52.096% inhibition at 500 mu g/ml. For lipid peroxidation assay, the dichloromethane, aqueous and n-hexane fractions of x moserianum showed 67.241, 66.147 and 64.213% inhibition respectively, while aqueous fraction of H. ericoides exhibited 67.404% inhibition at 500 mu g/ml. In cytotoxicity assay, all fractions of both the Species were found to be non-toxic on mouse fibroblast 3T3 cells with IC50 value greater than 30 mu g/ml as compared to cycloheximide with IC50 value 0.073 +/- 0.1 mu g/ml used as a standard. It was concluded from the study that among the two species, crude methanolic and ethyl acetate fractions were more active regarding the antioxidant, anti-glycation,activities while dichloromethane, aqueous and n-hexane fractions possessed anti-lipid peroxidation activity.