Disposable Glucose Concentration Detection Microfluidic Chip Fabricated by CO2 Laser Ablation

A novel 3D disposable glucose concentration detection chip is presented. The chip comprises a four-layer PMMA structure and is fabricated using a commercial CO2 laser and a hot-press bonding technique. In the proposed device, the glucose solution is injected into a double parallel connection micromixer (DPCM) and is mixed with DNS reagent by means of a self-rotation effect. The fluid streams exiting the two circular mixing chambers of the DPCM are then combined and mixed further at a T-type microchannel outlet before passing to a collection chamber. An experimental platform has been created for multiple reaction process. Numerical simulations are performed to analyze the vortex streamline distribution within the DPCM and to estimate the mixing performance. The numerical results show that a mixing efficiency as high as 92.5% can be obtained at low Reynolds numbers (Re=12). It is found a good linear relation of R-2=0.9953 from the chip detection method comparing to the traditional method of R-2=0.9976 at DNS reagent and glucose solution volume ratio of 1:1. In addition, the experimental results show that the accuracy of the glucose concentration measurements obtained using the proposed microfluidic chip is comparable with that of the measurements obtained using a conventional large-scale detection method. Overall, the results presented in this study indicate that the DPCM chip provides a rapid and low-cost means of detecting the concentration of glucose solutions.