Toxoplasma gondii in sheep from the Campania region (Italy)

被引：55

作者：

Fusco, Giovanna

Rinaldi, Laura

Guarino, Achille

Proroga, Yolande Therese Rose

Pesce, Antonella

Giuseppina, De Marco

Cringoli, Giuseppe

机构：

[1] Sez Caserta, Inst Zooprofilatt Sperimentale Mezzogiorno, I-81029 Tuoro, Caserta, Italy

[2] Univ Naples Federico 2, Fac Med Vet, Dipartimento Patol & Sanita Anim, I-80173 Naples, Italy

来源：

VETERINARY PARASITOLOGY | 2007年 / 149卷 / 3-4期

关键词：

Toxoplasma gondii; sheep; seroprevalence; PCR; milk;

D O I：

10.1016/j.vetpar.2007.07.020

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A cross-sectional serological survey was conducted in order to evaluate, irrespective of abortion, the Toxoplasma gondii infection in pastured sheep from the Campania region of southern Italy. A geographical information system was used in order to uniformly sample the ovine farms (n = 117) throughout the entire region. Blood and milk samples were collected from 10 adult sheeps (> 18 months) on each farm (total number = 1170 sheeps). Serum samples were tested for the presence of IgG antibodies to T gondii using a commercial indirect fluorescent antibody test. For each farm, the 10 milk samples collected were pooled in order to obtain a single milk sample per farm (total number = 117 milk samples). The 77.8% (91/117) of the farms and the 28.5% (333/ 11, 170) of the sheep resulted positive by serology. In addition, the presence of T gondii DNA was detected by PCR in 4 milk samples out of the 117 examined (3.4%). (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：271 / 274

页数：4

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