Elemental analysis of cellular samples by in-air micro-PIXE

被引：37

作者：

Ishii, K ^{[1
]}

Sugimoto, A

Tanaka, A

Satoh, T

Matsuyama, S

Yamazaki, H

Akama, C

Amartivan, T

Endoh, H

Oishi, Y

Yuki, H

Sugihara, S

Satoh, M

Kamiya, T

Sakai, T

Arakawa, K

Saidoh, M

Oikawa, S

机构：

[1] Tohoku Univ, Dept Quantum Sci & Energy Engn, Sendai, Miyagi 9808579, Japan

[2] Tohoku Univ, Dept Mechatron & Precis Engn, Sendai, Miyagi 9808579, Japan

[3] Takasaki Radiat Chem Res Estab, JAERI Takasaki, Gunma 3701292, Japan

[4] Ion Accelerator Corp, Hakodate, Hokkaido 0400076, Japan

来源：

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS | 2001年 / 181卷

关键词：

micro-PIXE analysis; quantitative micro-PIXE analysis; in-air PIXE; target preparation of cellular samples;

D O I：

10.1016/S0168-583X(01)00623-1

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

We developed a micro-PIXE system for the analysis of frozen cellular samples at atmospheric pressure under helium gas cooling. We analyzed bovine aortic endothelial (BAE) cells previously cultured in a medium containing bromodeoxyuridine (BrdU). It is known that these cells take BrdU into their nuclei at the S phase of their metabolic cycle. We examined the Br uptake for incubation times of 6, 12 and 24 h, respectively. The Br maps showed an accumulation in the nuclei and the Br concentration was found strongly dependent on the incubation time. We have demonstrated that in-air micro-PIXE analysis is a relevant technique for examining the molecular uptake in cells. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：448 / 453

页数：6

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