Elicitin-like proteins Oli-D1 and Oli-D2 from Pythium oligandrum trigger hypersensitive response in Nicotiana benthamiana and induce resistance against Botrytis cinerea in tomato

被引：27

作者：

Ouyang, Zhigang ^{[1
]}

Li, Xiaohui ^{[1
]}

Huang, Lei ^{[1
]}

Hong, Yongbo ^{[1
]}

Zhang, Yafen ^{[1
]}

Zhang, Huijuan ^{[1
]}

Li, Dayong ^{[1
]}

Song, Fengming ^{[1
]}

机构：

[1] Zhejiang Univ, Inst Biotechnol, Natl Key Lab Rice Biol, Hangzhou 310058, Zhejiang, Peoples R China

来源：

MOLECULAR PLANT PATHOLOGY | 2015年 / 16卷 / 03期

基金：

高等学校博士学科点专项科研基金;

关键词：

defence response/resistance; hypersensitive response; Nicotiana benthamiana; Oli-D1/Oli-D2; oligandrin; tomato; SYSTEMIC ACQUIRED-RESISTANCE; BACTERIAL WILT DISEASE; DEFENSE-RELATED GENES; HARPIN-ENCODING GENE; ORYZAE PV. ORYZICOLA; JASMONIC ACID; CELL-DEATH; ARABIDOPSIS-THALIANA; MAGNAPORTHE-GRISEA; SIGNALING PATHWAYS;

D O I：

10.1111/mpp.12176

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The biocontrol agent Pythium oligandrum and its elicitin-like proteins oligandrins have been shown to induce disease resistance in a range of plants. In the present study, the ability of two oligandrins, Oli-D1 and Oli-D2, to induce an immune response and the possible molecular mechanism regulating the defence responses in Nicotiana benthamiana and tomato were investigated. Infiltration of recombinant Oli-D1 and Oli-D2 proteins induced a typical immune response in N. benthamiana including the induction of a hypersensitive response (HR), accumulation of reactive oxygen species and production of autofluorescence. Agrobacterium-mediated transient expression assays revealed that full-length Oli-D1 and Oli-D2 were required for full HR-inducing activity in N. benthamiana, and virus-induced gene silencing-mediated knockdown of some of the signalling regulatory genes demonstrated that NbSGT1 and NbNPR1 were required for Oli-D1 and Oli-D2 to induce HR in N. benthamiana. Subcellular localization analyses indicated that both Oli-D1 and Oli-D2 were targeted to the plasma membrane of N. benthamiana. When infiltrated or transiently expressed in leaves, Oli-D1 and Oli-D2 induced resistance against Botrytis cinerea in tomato and activated the expression of a set of genes involved in the jasmonic acid/ethylene (JA/ET)-mediated signalling pathway. Our results demonstrate that Oli-D1 and Oli-D2 are effective elicitors capable of inducing immune responses in plants, probably through the JA/ET-mediated signalling pathway, and that both Oli-D1 and Oli-D2 have potential for the development of bioactive formulae for crop disease control in practice.

引用

页码：238 / 250

页数：13